Genetic Characteristics and Multiple-PCR Development for Capsular Identification of Specific Serotypes of Campylobacter jejuni
نویسندگان
چکیده
منابع مشابه
Genetic Characteristics and Multiple-PCR Development for Capsular Identification of Specific Serotypes of Campylobacter jejuni
The polysaccharide capsule (CPS) of Campylobacter jejuni is a virulence factor linked to cell surface carbohydrate diversity which mainly determines the serotypes. Thirty-four CPS gene cluster structures have been published and some of them can be distinguished by multiple-PCR. Penner serotypes HS1/44c, HS2, HS4c, HS19, HS23/36c and HS41 are markers for Guillain-Barré syndrome (GBS). The capsul...
متن کاملidentification of campylobacter jejuni and campylobacter coli from diarrheic samples using pcr
background: campylobacter jejune and c. coli are recognized as the most common bacteriological causes of gastroenteritis in humans. in this study identification of campylobacter jejuni and campylobacter coli from samples using pcr was explored. methods: detection was performed using diarrheal samples collected 8 from 117 children. the genomic dna of samples was extracted by phenol-chloroform me...
متن کاملA specific DNA probe for the identification of Campylobacter jejuni.
A 6.1 kb DNA probe for the human enteric pathogen Campylobacter jejuni has been isolated from a genomic library constructed in the plasmid vector pBR322 in Escherichia coli. The DNA sequence used as a probe was identified from recombinant plasmids following immunological screening of transformants using polyclonal antisera to whole cells and to membrane antigens of C. jejuni. Restriction endonu...
متن کاملSpecific identification, grouping and differentiation of Campylobacter jejuni among thermophilic campylobacters using multiplex PCR.
Campylobacter species such as C. jejuni and C. coli are recognized as major causes of acute gastroenteritis world-wide. Although C. jejuni and C. coli are usually non-pathogenic in birds and animals, they cause enteric disease in humans and the source of infection is often the consumption of contaminated foodstuffs. In this paper we report on the development and use of a multiplex PCR of C. jej...
متن کاملin silico analysis of the cadf gene and development of a duplex polymerase chain reaction for species-specific identification of campylobacter jejuni and campylobacter coli
materials and methods in silico analysis of the cadf gene was carried out by several software and available online tools. a duplex pcr optimized with specific primers was used for detection and differentiation of both species. to evaluate specificity and sensitivity of the test, a panel of different campylobacter spp. together with several intestinal bacterial pathogens was tested. the limit of...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2016
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0165159